| Product Name |
CHO-K1/V2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant V2 Vasopressin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Arginine vasopressin (AVP) is a cyclic nonapeptide that acts by binding to a family of vasopressin receptors that includes V1a, V1b, and V2 receptors. In particular, V2 receptors are expressed in kidney where vasopressin exerts its antidiuretic action. V1a and V1b couple to Gq and calcium release, whereas V2 couples to Gs. Mutations in V2 result in X-linked nephrogenic diabetes insipidus, a syndrome in which the kidney is unable to concentrate urine, leading to dehydration and hypernatremia. Conversely, elevated levels of AVP lead to hyponatremia in the syndrome of inappropriate antidiuretic hormone secretion (SIADH), congestive heart failure or cirrhosis, and V2 selective antagonists have been developed to treat these conditions. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for V2 receptor |
Application Examples |
Figure 1. AVP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/V2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a V2 receptor agonist, AVP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of AVP (Mean ± SD, n = 2). The EC50 of AVP on V2 co-expressing with Gα15 in CHO-K1 cells was 24 nM. The S/B of AVP on V2 co-expressing with Gα15 in CHO-K1 cells was 25. |
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