| Product Name |
CHO-K1/M1 Stable Cell Line |
Full Name |
Human Recombinant Muscarinic Acetylcholine Receptor M1 Stable Cell Line |
|
Species |
Human |
Description |
M1 was expressed in the CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system and esophageal smooth muscle. Synaptic transmission by muscarinic acetylcholine receptors (mAChRs) is employed throughout the central and peripheral nervous systems to elicit a large and diverse array of neurophysiological actions. An important aspect of mAChR functional diversity is reflected by the multitude of biochemical and electrophysiological actions evoked by acetylcholine binding to mAChRs, which include the regulation of intracellular levels of cAMP, cGMP and inositol phospholipids, and the opening or closing of the potassium, calcium, and chloride ion channels found in certain tissues. |
Host Cell |
CHO-K1 |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, and 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for M1 receptor |
Application Examples |
Figure 1. Carbachol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M1 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an M1 receptor agonist, Carbachol. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Carbachol (Mean ± SD, n = 2). The EC50 of Carbachol on M1 in CHO-K1 cells was 0.44 nM. The S/B of Carbachol on M1 in CHO-K1 cells was 76. |
Related Products
 |
1 |
Gene Synthesis: Custom gene synthesis service starts at as LOW as $0.35/bp, with advanced OptimumGeneTM codon optimization technology and CloneEZ® seamless cloning technology. (starting from $0.29/bp for non-codon optimized human, mouse and rat gene sequences) |
2 |
PCR Cloning and Subcloning: Order GenScript's flexible PCR cloning and subcloning service RIGHT NOW to get fully validated clones delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: Special mutagenesis bundle starts at $149/mutation. Site-directed Mutagenesis services include point mutations, deletions, and insertions for unparalleled accuracy, unlimited sites and comprehensive, fully validated deliverables. |
4 |
Plasmid DNA Preparation: Endotoxin FREE plasmid DNA preparation services deliver predominantly supercoiling plasmid DNA ranging from 100 ug to 1000 mg starting from $45. |
|
TECHNICAL MANUAL: 20090427231334 (PDF)
