| Product Name |
CHO-K1/GHSR Stable Cell Line |
Full Name |
Human Recombinant Growth Hormone Secretagogue Receptor Stable Cell Line |
|
Species |
Human |
Description |
Ghrelin, a 28-amino-acid gut peptide is secreted from the stomach. It stimulates the release of growth hormone (GH) from the pituitary through GHS-R and plays important roles in energy homeostasis. In addition, ghrelin acts directly on the central nervous system to decrease sympathetic nerve activity. Ghrelin receptors (GHSRs) are concentrated in the hypothalamus-pituitary unit and may take the role of ghrelin in memory. GHSR is distributed in peripheral tissues, including the heart, lung, liver, kidney, pancreas, stomach, small and large intestines, adipose, and immune cells, suggesting that ghrelin has multiple functions in these tissues. |
Freeze Medium |
45% culture medium,45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 500 µg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for GHSR receptor |
Application Examples |
Figure 1. Ghrelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GHSR and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a GHSR receptor agonist, Ghrelin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Ghrelin (Mean ± SD, n = 2). The EC50 of Ghrelin on GHSR in CHO-K1 cells was 5.3 nM. The S/B of Ghrelin on GHSR in CHO-K1 cells was 9. |
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