| Product Name |
CHO-K1/KiSS1 Stable Cell Line |
Full Name |
Human Recombinant KiSS1-derived Peptide Receptor Stable Cell Line |
|
Species |
Human |
Description |
The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a Gq-coupled receptor which binds the peptide hormone kisspeptin (metastin). The KiSS1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS1 in inhibition of metastasis in human cancer. Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus. In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for KiSS1 receptor |
Application Examples |
Figure 1. Metastin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/KiSS1 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a KiSS1 receptor agonist, Metastin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Metastin (Mean ± SD, n = 2). The EC50 of Metastin on KiSS1 in CHO-K1 cells was 7.6 nM. The S/B of Metastin on KiSS in CHO-K1 cells was 12. |
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TECHNICAL MANUAL: 13136_20100719032150.PDF (PDF)
Document-EXAMPLE: 11762_20100131205917.JPG (JPG)