| Product Name |
CHO-K1/OXTR Stable Cell Line |
Full Name |
Human Recombinant Oxytocin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Oxytocin is an abundant neuropeptide that exerts a wide spectrum of central and peripheral effects. In the context of human reproduction, oxytocin promotes uterine contractions and lactation. It is the most commonly used drug for labor induction. Oxytocin receptors are expressed in the uterus and in mammary glands, where they mediate functions related to partutition, such as contraction of the uterine myometrium during labor and milk letdown. In addition, oxytocin receptors are expressed in a variety of other peripheral tissues and in the brain, where oxytocin mediates a variety of functions. OXTR antagonists may be promising candidates to prevent preterm labor and dysmenorrheal and its agonists may also be useful for treatment of psychiatric illnesses such as anxiety, drug abuse, sexual dysfunctions, eating disorders, and autism. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for OXTR receptor |
Application Examples |
Figure 1. Oxytocin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OXTR and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an OXTR receptor agonist, Oxytocin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Oxytocin (Mean ± SD, n = 2). The EC50 of Oxytocin on OXTR in CHO-K1 cells was 0.0087 nM. The S/B of Oxytocin on OXTR in CHO-K1 cells was 11. |
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TECHNICAL MANUAL: 10008_20090629231122.PDF (PDF)
Document-EXAMPLE: 10010_20090629231343.JPG (JPG)