| Product Name |
CHO-K1/NK1 Stable Cell Line |
Full Name |
Human Recombinant NK1 Tachykinin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Tachykinins are peptides sharing the common C-terminal amino acid sequence Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. It plays a role as both a neurotransmitter and a neuromodulator. Their actions are mediated by binding with three distinct receptors, namely, NK1, NK2, and NK3. NK1 has high affinity with substance P. In the CNS, NK1 has been implicated to play a role in regulating neuronal survival and degeneration. In the cardiovascular system, NK1 mediates endothelium-dependent vasodilatation and plasma protein extravasations. In the gastrointestinal system, NK1 receptors mediate intestinal motility, secretion, and vascular functions. SP-NK1 receptor communication is also involved in glioma development and progression. NK1 receptor antagonists may have several therapeutic applications in diseases mediated by tachykinins, such as pulmonary disorders, gut disorders, and the pathophysiology of depression. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for NK1 receptor |
Application Examples |
Figure 1. Substance P-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK1 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK1 receptor agonist, Substance P. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Substance P (Mean ± SD, n = 2). The EC50 of Substance P on NK1 in CHO-K1 cells was 0.22 nM. The S/B of Substance P on NK1 in CHO-K1 cells was 39. |
Related Products
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
|
Document-EXAMPLE: 9780_20090520050809.JPG (JPG)
TECHNICAL MANUAL: 9800_20090522014129.PDF (PDF)