| Product Name |
CHO-K1/NK3 Stable Cell Line |
Full Name |
Human Recombinant NK3 Tachykinin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Tachykinins are peptides sharing a common C-terminal amino acid sequence: Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. These three molecules serve as both neurotransmitters and neuromodulators. Their actions are mediated by binding with three distinct receptors, namely NK1, NK2, and NK3. NK3 receptors show affinity for neurokinin B. They are predominantly expressed in both the peripheral and central nervous systems. NK3 receptors appear to modulate monoaminergic and amino acid neurotransmission. Studies show that manipulating modulation of NK3 receptor activity may have therapeutic utility in psychiatric diseases such as schizophrenia and affective disorders. |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for NK3 receptor |
Application Examples |
Figure 1. Neurokinin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK3 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK3 receptor agonist, Neurokinin B. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Neurokinin B (Mean ± SD, n = 2). The EC50 of Neurokinin B on NK3 in CHO-K1 cells was 4.6 nM. The S/B of Neurokinin B on NK3 in CHO-K1 cells was 18. |
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