| Product Name |
CHO-K1/V1B Stable Cell Line |
Full Name |
Human Recombinant V1B Vasopressin Receptor Stable Cell Line |
|
Species |
Human |
Description |
The antidiuretic hormone arginine vasopressin (AVP) receptors are G protein-coupled receptors which consists of at least three types: V1A (vascular/hepatic) and V1B (anterior pituitary) receptors, which act through phosphatidylinositol hydrolysis to mobilize intracellular Ca2+; and V2 (kidney) receptor, which is coupled to adenylate cyclase. V1B receptors are expressed in anterior pituitary where they mediate the release of ACTH . Its peripheral actions, such as antidiuresis, contraction of vascular smooth muscle, and stimulation of hepatic glycogenolysis are well characterized. |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for V1B receptor |
Application Examples |
Figure 1. AVP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/V1B and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a V1B receptor agonist, AVP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of AVP (Mean ± SD, n = 2). The EC50 of AVP on V1B in CHO-K1 cells was 0.12 nM. The S/B of AVP on V1B in CHO-K1 cells was 46. |
Related Products
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
|
Document-EXAMPLE: 9777_20090520040910.JPG (JPG)
TECHNICAL MANUAL: 9836_20090524233630.PDF (PDF)