CHO-K1/GAL2 Stable Cell Line

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Product Name

Full Name

Human Recombinant GAL2 Galanin Receptor Stable Cell Line

Documents
Document-EXAMPLE: 9785_20090520222311.JPG (JPG)
TECHNICAL MANUAL: 9790_20090521221128.PDF (PDF)
Document-MSDS: 10394_20090813065723.PDF (PDF)
Figures
Reference

Species

Human

Description

Actions of neuropeptide galanin are mediated by three G-protein coupled receptors, GalR1, GalR2, and GalR3. Human GALR2 was shown to couple to phospholipase C and to be involved in the elevation of intracellular calcium levels. GALR2 mRNA shows a wide distribution in the brain (mammillary nuclei, dentate gyrus, cingulate gyrus, posterior hypothalamic, supraoptic, and arcuate nuclei) and restricted peripheral tissue distribution with highest mRNA levels detected in human small intestine. Galanin has been implicated in anxiety-related behaviors, cognition, analgesia, and feeding in rodents.

Freeze Medium

45% culture medium, 45% FBS, and 10% DMSO

Culture Medium

Ham's F12, 10% FBS, 400 μg/ml G418

Storage

Liquid nitrogen immediately upon delivery

Application

Functional assays for GAL2 receptor

Application Examples

Figure 1. Galanin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GAL2 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a GAL2 receptor agonist, Galanin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Galanin (Mean ± SD, n = 2). The EC50 of Galanin on GAL2 in CHO-K1 cells was 47 nM. The S/B of Galanin on GAL2 in CHO-K1 cells was 10.

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