| Product Name |
CHO-K1/OX1 Stable Cell Line |
Full Name |
Human Recombinant OX1 Orexin Receptor Stable Cell Line |
|
Species |
Human |
Description |
The orexin/hypocretin peptides orexin A and orexin B (also known as hcrt-1 and hcrt-2) are 33- and 28-amino acid peptides, respectively. They are preferentially expressed in hypothalamus. The orexins have a range of physiological functions including feeding control, energy metabolism, neuroendocrine function modulation, and sleep-wake cycle regulation. The two orexin receptor subtypes OX1 and OX2 both mediate the action of orexin-A and orexin-B and couple efficiently through Gq/11 to activate phospholipase C and lead to elevation of intracellular calcium. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418
|
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for OX1 receptor |
Application Examples |
Figure 1. Orexin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OX1 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a OX1 receptor agonist, Orexin A. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Orexin A (Mean ± SD, n = 2). The EC50 of Orexin A on OX1 in CHO-K1 cells was 22 nM. The S/B of Orexin A on OX1 in CHO-K1 cells was 30. |
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