| Product Name |
CHO-K1/ADRA1A Stable Cell Line |
Full Name |
Human Recombinant ADRA1A Adrenoceptors Stable Cell Line |
|
Species |
Human |
Description |
The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D) that mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains predicted to have seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase both inositol 1,4,5-trisphosphate production and intracellular Ca2+. Once activated by binding, α1-ARs initiate the cellular pathways leading to the regulation of physiological effects, including blood pressure maintenance, glucose metabolism, renal sodium reabsorption, and cardiac inotropy. |
Host Cell |
CHO-K1 |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 µg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADRA1A receptor |
Application Examples |
Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1A and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADRA1A receptor agonist, Epinephrine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on ADRA1A in CHO-K1 cells was 1.3 nM. The S/B of Epinephrine on ADRA1A in CHO-K1 cells was 45. |
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1 |
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MSDS: 20090818212041 (PDF)
