| Product Name |
CHO-K1/VPAC2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant VPAC2 Receptor Stable Cell Line |
|
Species |
Human |
Description |
The Vasoactive intestinal polypeptide receptor 2 (VPAC2) is a Gs-coupled receptor expressed in the stroma of uterus and prostate; smooth muscles in gastrointestinal tract, seminal vesicles and skin; blood vessels; thymus. VPAC2 is an essential regulator of the circadian pacemaker of the hypothalamic suprachiasmatic nuclei. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for VPAC2 receptor |
Application Examples |
Figure 1. VIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/VPAC2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a VPAC2 receptor agonist, VIP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of VIP (Mean ± SD, n = 2). The EC50 of VIP on VPAC2 co-expressing with Gα15 in CHO-K1 cells was 160 nM. The S/B of VIP on VPAC2 co-expressing with Gα15 in CHO-K1 cells was 7. |
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TECHNICAL MANUAL: 13138_20100719032648.PDF (PDF)
Document-EXAMPLE: 13141_20100719032935.JPG (JPG)