| Product Name |
CHO-K1/NPY4/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Neuropeptide Y Receptor Y4 Stable Cell Line |
|
Species |
Human |
Description |
The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. Y4 receptor preferentially binds PP, with significant binding to NPY and PYY. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for NPY4 receptor |
Application Examples |
Figure 1. Pancreatic Polypeptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPY4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a NPY4 receptor agonist, Pancreatic Polypeptide. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Pancreatic Polypeptide (Mean ± SD, n = 2). The EC50 of Pancreatic Polypeptide on NPY4 co-expressing with Gα15 in CHO-K1 cells was 31 nM. The S/B of Pancreatic Polypeptide on NPY4 co-expressing with Gα15 in CHO-K1 cells was 4. |
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