molecule » GenScript Stable Cell Line: CHO-K1/M4/Gα15 Stable Cell Line
Cat. No.
Name
Size   
Price
Selected
M00238
2 vials
$11,600
Product Name CHO-K1/M4/Gα15 Stable Cell Line
Full Name
Human Recombinant Muscarinic Acetylcholine Receptor M4 Stable Cell Line
Documents
TECHNICAL MANUAL: 12909_20100610033339.PDF (PDF)
Document-MSDS: 13188_20100813045049.PDF (PDF)
Figures
CHO-K1/M4/Gα15 Stable Cell Line zoomCHO-K1/M4/Gα15 Stable Cell Line
Species
Human
Description
Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M4 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M4 with Gα15 in the CHO-K1 which supports high levels of recombinant M4 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway.
Freeze Medium
45% culture medium, 45% FBS, and 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assays for M4 receptor
Application Examples
Figure 1. Oxotremorine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an M4 receptor agonist, Oxotremorine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Oxotremorine (Mean ± SD, n = 2). The EC50 of Oxotremorine on M4 co-expressing with Gα15 in CHO-K1 cells was 14 nM. The S/B of Oxotremorine on M4 co-expressing with Gα15 in CHO-K1 cells was 28.
Document-COA
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