| Product Name |
293T/NMU2 Stable Cell Line |
Full Name |
Human Recombinant Neuromedin U Receptor 2 Stable Cell Line |
|
Species |
Human |
Description |
NMU1 and NMU2 are two G-protein coupled receptors binding to Neuromedin U (NmU) that is a peptide which regulates peripheral functions such as smooth muscle contraction and blood pressure, and CNS functions including nociception and feeding activity. Compared to the wide distribution of NMU1 in peripheral tissue, expression of NMU2 receptor is limited to areas of the brain, such as the paraventricular nucleus, along the wall of the third ventricle in the hypothalamus and the CA1 region of the hippocampus, and to the spinal cord. |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 100 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for NMU2 receptor |
Application Examples |
Figure 1. NMU25-induced concentration-dependent stimulation of intracellular calcium mobilization in 293T/NMU2 and 293T cells. The cells were loaded with Calcium-4 prior to stimulation with a NMU2 receptor agonist, NMU25. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of NMU25 (Mean ± SD, n = 2). The EC50 of NMU25 on NMU2 in 293T cells was 22 nM. The S/B of NMU25 on NMU2 in 293T cells was 8. |
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