CHO-K1/D1 Stable Cell Line

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4).

Price	$8500.00
Cat. No.	M00247
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human dopamine receptor D1 (D1) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

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Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with dopamine in CHO-K1/D1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/D1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of dopamine on CHO-K1/D1 cells was 53.05 nM.

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Citations

1. Mi G, et al. Levo-Tetrahydroberberrubine Produces Anxiolytic-Like Effects in Mice through the 5-HT1A Receptor. PLoS One. (2017-01)