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Cat. No.
Product Name
M00249 CHO-K1/5-HT2B Stable Cell Line
2 vials
Product Name CHO-K1/5-HT2B Stable Cell Line
CHO-K1/5-HT2B Stable Cell LineDocument-MSDS: 10461_20090818222426.PDF (PDF)
CHO-K1/5-HT2B Stable Cell LineTECHNICAL MANUAL: M00249_Manual.pdf (pdf)
CHO-K1/5-HT2B Stable Cell Line zoomCHO-K1/5-HT2B Stable Cell Line
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2B which couples to Gq/11 is expressed in embryonic and adult cardiovascular tissues, gut, and brain from the rat, mouse, and human species. 5-HT2B receptors are responsible for many cardiovascular and central nervous system functions, such as blood vessel contraction, platelet shape changes, neuronal sensitization to tactile stimuli, and mediation of the hallucinogenic effects of phenylisopropylamin hallucinogens. It has also been shown to be required for heart development. GenScript's cloned human 5-HT2B -expressing cell line is generated in the CHO-K1 host.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin
Functional assay for 5-HT2B receptor
Application Examples
Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2B and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a 5-HT2B receptor agonist, 5-HT. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on 5-HT2B in CHO-K1 cells was 0.45 nM. The S/B of 5-HT on 5-HT2B in CHO-K1 cells was 6.
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