Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/5-HT2B
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Cat. No.
Name
Size   
Price
Selected
M00249
2 vials
$8,500
Product Name CHO-K1/5-HT2B Stable Cell Line
Full Name
Human Recombinant 5-HT2B Serotonin Receptor Stable Cell Line
Documents
Document-MSDS: 10461_20090818222426.PDF (PDF)
TECHNICAL MANUAL: M00249_Manual.pdf (pdf)
Figures
CHO-K1/5-HT2B Stable Cell Line zoomCHO-K1/5-HT2B Stable Cell Line
Species
Human
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin
Application
Functional assay for 5-HT2B receptor
Application Examples
Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2B and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a 5-HT2B receptor agonist, 5-HT. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on 5-HT2B in CHO-K1 cells was 0.45 nM. The S/B of 5-HT on 5-HT2B in CHO-K1 cells was 6.
Document-COA
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