| Product Name |
1321N1/P2Y1 Stable Cell Line |
Full Name |
Human Recombinant P2Y1 Purinergic Receptor Stable Cell Line |
|
Species |
Human |
Description |
P2Y receptors are members of a large family of G protein-coupled receptors that is classified into five functionally characterized human gene products (P2Y1, P2Y2, P2Y4, P2Y6, and P2Y11). P2RY1 or P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. P2Y1 express on vascular endothelium and smooth muscle, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for P2Y1 receptor |
Application Examples |
Figure 1. ADP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y1 and 1321N1 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y1 receptor agonist, ADP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of ADP (Mean ± SD, n = 2). The EC50 of ADP on P2Y1 in 1321N1 cells was 58 nM. The S/B of ADP on P2Y1 in 1321N1 cells was 14. |
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