| Product Name |
CHO-K1/5-HT2A Stable Cell Line |
Full Name |
Human Recombinant 5-HT2A Serotonin Receptor Stable Cell Line |
|
Species |
Human |
Description |
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2A which couples to Gq/11 is expressed throughout the central nervous system in the neocortex and olfactory tubercle. 5-HT2A receptor agonists may have important clinical value in the treatment of various disorders, such as depression, anxiety, bipolar disorder, and schizophrenia. GenScript's cloned human 5-HT2Aâexpressing cell line is generated in the CHO-K1 host. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for 5-HT2A receptor |
Application Examples |
Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2A and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a 5-HT2A receptor agonist, 5-HT. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on 5-HT2A in CHO-K1 cells was 1.9 nM. The S/B of 5-HT on 5-HT2A in CHO-K1 cells was 222. |
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Document-MSDS: 10462_20090818222528.PDF (PDF)
Document-EXAMPLE: 10560_20090826045705.JPG (JPG)