| Product Name |
CHO-K1/BB1 Stable Cell Line |
Full Name |
Human Recombinant BB1 Bombesin Receptor Stable Cell Line |
|
Species |
Human |
Description |
The bombesin receptor family is a member of the G protein-coupled receptor superfamily and consists of three subtypes of receptors: BB1 (neuromedin B receptor), BB2 (gastrin-releasing peptide receptor), and BB3. These receptors are widely distributed in mammals especially in the CNS and gastrointestinal tract and have numerous effects both physiologically and in pathologic processes including having an autocrine growth action on normal tissues and tumors; potent CNS effects (satiety, circadian rhythm, various behaviors); and potent effects in the immune, gastrointestinal, respiratory and urogenital systems.
The human BB1 receptor has a greater than 100-fold higher affinity for neuromedin B than gastrin-releasing peptide. Interaction of ligand and BB1 activates the Gαq/11 pathway and increase the intracellular calcium concentration. GenScript's BB1-expressing stable cell line was made in CHO-K1 host cell and optimized for calcium assays. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for BB1 receptor |
Application Examples |
Figure 1. Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB1 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB1 receptor agonist, Neuromedin B. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Neuromedin B (Mean ± SD, n = 2). The EC50 of Neuromedin B on BB1 in CHO-K1 cells was 0.025 nM. The S/B of Neuromedin B on BB1 in CHO-K1 cells was 7. |
Related Products
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
|
Document-EXAMPLE: 12959_20100617213002.JPG (JPG)
TECHNICAL MANUAL: 12988_20100627230605.PDF (PDF)