| Product Name |
CHO-K1/M3 Stable Cell Line |
Full Name |
Human Recombinant Muscarinic Acetylcholine Receptor M3 Stable Cell Line |
|
Species |
Human |
Description |
Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M3 muscarinic receptors are located at many places in the body, e.g. smooth muscles, endocrine, exocrine glands, as well as lungs. They are also found in the CNS, where it induces emesis. They generally cause smooth muscle contraction and increased glandular secretions. |
Host Cell |
CHO-K1 |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Hams F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for M3 receptor |
Application Examples |
Figure 1. Carbachol-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M3 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an M3 receptor agonist, Carbachol. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Carbachol (Mean ± SD, n = 2). The EC50 of Carbachol on M3 in CHO-K1 cells was 3.4 nM. The S/B of Carbachol on M3 in CHO-K1 cells was 76. |
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TECHNICAL MANUAL: 20090630014401 (PDF)
