| Product Name |
CHO-K1/ADRA1B Stable Cell Line |
Full Name |
Human Recombinant ADRA1B Adrenoceptors Stable Cell Line |
|
Species |
Human |
Description |
The α1-adrenergic receptor (AR) family consists of three closely related gene products (α1A, α1B, and α1D). They mediate the actions of norepinephrine (NE) and epinephrine in sympathetically innervated tissues and brain. Α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains that are predicted to form seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase inositol 1,4,5-trisphosphate production, and increase intracellular Ca2+. ADRA1B functions in diverse settings include vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, as well as neuroendocrine regulation of fertility. |
Host Cell |
CHO-K1 |
Freeze Medium |
45% culture medium, 45%FBS, and 10%DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for ADRA1B receptor
|
Application Examples |
Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1B and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a ADRA1B receptor agonist, Epinephrine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on ADRA1B in CHO-K1 cells was 44 nM. The S/B of Epinephrine on ADRA1B in CHO-K1 cells was 19. |
 |
1 |
Gene Synthesis: Custom gene synthesis service starts at as LOW as $0.35/bp, with advanced OptimumGeneTM codon optimization technology and CloneEZ® seamless cloning technology. (starting from $0.29/bp for non-codon optimized human, mouse and rat gene sequences) |
2 |
PCR Cloning and Subcloning: Order GenScript's flexible PCR cloning and subcloning service RIGHT NOW to get fully validated clones delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: Special mutagenesis bundle starts at $149/mutation. Site-directed Mutagenesis services include point mutations, deletions, and insertions for unparalleled accuracy, unlimited sites and comprehensive, fully validated deliverables. |
4 |
Plasmid DNA Preparation: Endotoxin FREE plasmid DNA preparation services deliver predominantly supercoiling plasmid DNA ranging from 100 ug to 1000 mg starting from $45. |
|
TECHNICAL MANUAL: 20090630015950 (PDF)
