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*This product is under development*
| Product Name |
CHO-K1/GPR40-GFP |
Full Name |
Human Recombinant GPR40 Stable Cell Line |
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Species |
Human |
Description |
Free fatty acid G protein coupled receptor family consists of four members and plays significant roles in nutrition regulation. GPR40 and GPR120 are activated by medium and long-chain FFAs, whereas GPR41 and GPR43 are activated by short-chain FFAs. GPR40, preferentially expressed in pancreatic beta-cells, mediates the majority of the effects of FFAs on insulin secretion. Research findings have shown that GPR40 as a potential therapeutic target plays an important role in the chain of events linking obesity and type2 diabetes. |
Freeze Medium |
45% culture medium, 45% FBS, and 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for GPR40 receptor |
Application Examples |
Figure: Shown above are the intracellular calcium responses of CHO-K1 cells stably expressing Homo sapiens free fatty acid receptor 1 (FFA1/GPR40) and of untransfected control cells. Cells were loaded with Calcium-4 and then stimulated with the indicated concentrations of Oleic acid. Calcium responses were recorded on a FlexStation plate reader. Data represent the average +/- standard deviation of triplicate determinations. |
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TECHNICAL MANUAL: 9971_20101012045901.PDF (PDF)
Document-EXAMPLE: 9972_20090618024738.JPG (JPG)