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Cat. No.
Product Name
M00269 CHO-K1/ADRB1/Gα15 Stable Cell Line
2 vials
Product Name CHO-K1/ADRB1/Gα15 Stable Cell Line
CHO-K1/ADRB1/Gα15 Stable Cell LineTECHNICAL MANUAL: M00269_Manual.pdf (pdf)
CHO-K1/ADRB1/Gα15 Stable Cell LineDocument-MSDS: 10401_20090813070445.PDF (PDF)
CHO-K1/ADRB1/Gα15 Stable Cell Line zoomCHO-K1/ADRB1/Gα15 Stable Cell Line
The ß-adrenergic receptors are linked to G proteins. The ß-receptor has three known subtypes. Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction. Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation. Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd,2006). In the human heart, beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance. Changes in betaAR play an important role in chronic heart failure (CHF). Thus, due to increased sympathetic activity in CHF, betaAR are chronically (over) stimulated, and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE,2006).
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Liquid nitrogen immediately upon delivery
Functional assay for ADRB1 receptor
Application Examples
Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRB1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADRB1 receptor agonist, Epinephrine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on ADRB1 co-expressing with Gα15 in CHO-K1 cells was 240 nM. The S/B of Epinephrine on ADRB1 co-expressing with Gα15 in CHO-K1 cells was 17.
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