293/PTH2/CRE-Luc Stable Cell Line

*This product has been discontinued!*

Recombinant HEK293 cells stably overexpress human parathyroid hormone 2 receptor (PTH2) on the surface and contain high levels of G protein Gαs and Gαq to couple with the receptor in downstream signaling pathways.

Cat. No.	M00270
Size	Buy in bulk

Ask us a question

Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant HEK293 cells stably overexpress human parathyroid hormone 2 receptor (PTH2) on the surface and contain high levels of G protein Gαs and Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Back

Culture Conditions

Culture Medium	DMEM, 10% FBS, 300 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 50 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen), 100 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium	Complete Growth Medium: DMEM, 10% FBS.
Freeze Medium-DATA	50% DMEM (Cat. No. 10566, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma).

Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.