| Product Name |
CHO-K1/D2s/Gα15 Stable Cell Line |
Full Name |
Human Recombinant D2 (Short) Dopamine Receptor Stable Cell Line |
|
Species |
Human |
Description |
The short form of D2 (D2S) and the long form (D2L) are two isoforms that contribute differentially to dopamine signaling in both prefrontal cortex and striatum. The D2 dopamine receptor, short form (D2s) has been shown to stimulate phospholipase D (PLD) activity independent of the activation of phospholipase C (PLC) activity in GH4 derived cells stably transfected with the D2s receptor. Agonist activation of D2s has been shown to mediate the inhibition of growth in the same cell line. |
Freeze Medium |
45% culture medium, 45% FBS,10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for D2s receptor |
Application Examples |
Figure 1. Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2S/Gα15 and CHO-K1/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a D2S receptor agonist, Dopamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Dopamine (Mean ± SD, n = 2). The EC50 of Dopamine on D2S co-expressing with Gα15 in CHO-K1 cells was 1.7 nM. The S/B of Dopamine on D2S co-expressing with Gα15 in CHO-K1 cells was 7. |
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