Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/GAL1/Gα15
Cat. No.
Name
Size   
Price
Selected
M00277
2 vials
$8,500
Product Name CHO-K1/GAL1/Gα15 Stable Cell Line
Full Name
Human Recombinant GAL1 Galanin Receptor Stable Cell Line
Documents
Document-MSDS: 10465_20090818222812.PDF (PDF)
TECHNICAL MANUAL: M00277_Manual.pdf (pdf)
Figures
CHO-K1/GAL1/Gα15 Stable Cell Line zoomCHO-K1/GAL1/Gα15 Stable Cell Line
Species
Human
Description
The neuropeptide galanin elicits a range of biological effects by interacting with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins that have been shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain, spinal cord, and peripheral sites including small intestine and heart.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, and 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application Examples
Figure 1. Galanin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GAL1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GAL1 receptor agonist, Galanin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Galanin (Mean ± SD, n = 2). The EC50 of Galanin on GAL1 co-expressing with Gα15 in CHO-K1 cells was 34 nM. The S/B of Galanin on GAL1 co-expressing with Gα15 in CHO-K1 cells was 5.
Document-COA
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