| Product Name |
CHO-K1/GAL1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant GAL1 Galanin Receptor Stable Cell Line |
|
Species |
Human |
Description |
The neuropeptide galanin elicits a range of biological effects by interacting with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins that have been shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain, spinal cord, and peripheral sites including small intestine and heart. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, and 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application Examples |
Figure 1. Galanin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GAL1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GAL1 receptor agonist, Galanin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Galanin (Mean ± SD, n = 2). The EC50 of Galanin on GAL1 co-expressing with Gα15 in CHO-K1 cells was 34 nM. The S/B of Galanin on GAL1 co-expressing with Gα15 in CHO-K1 cells was 5. |
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