Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/ADRA2A/Gα15 Stable Cell Line
Cat. No.
Name
Size   
Price
Selected
M00281
2 vials
$11,600
Product Name CHO-K1/ADRA2A/Gα15 Stable Cell Line
Full Name
Human Recombinant ADRA2A Adrenoceptors Stable Cell Line
Documents
TECHNICAL MANUAL: M00281_Manual.pdf (pdf)
Document-MSDS: 13202_20100813051533.PDF (PDF)
Figures
CHO-K1/ADRA2A/Gα15 Stable Cell Line zoomCHO-K1/ADRA2A/Gα15 Stable Cell Line
Species
Human
Description
The adrenoceptors ADRA2A is expressed in the brain, spleen, kidney, aorta, lung, skeletal muscle, heart and liver. ADRA2A -adrenoceptor knockout mice exhibit disruption of presynaptic inhibition of noradrenaline release at high stimulation frequencies. ADRA2A receptor is the principal autoreceptor in the presynaptic feedback loop regulating noradrenaline release. However, another α2 autoreceptor is also present.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham’s F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for ADRA2A receptor
Application Examples
Figure 1. UK140304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2A/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADRA2C receptor agonist, UK140304. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of UK140304 (Mean ± SD, n = 2). The EC50 of UK140304 on ADRA2A co-expressing with Gα15 in CHO-K1 cells was 0.53 nM. The S/B of ACTH on ADRA2A co-expressing with Gα15 in CHO-K1 cells was 9.
Document-COA
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