Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/H4/Gα15
Cat. No.
Name
Size   
Price
Selected
M00289
2 vials
$11,600
Product Name CHO-K1/H4/Gα15 Stable Cell Line
Full Name
Human Recombinant H4 Histamine Receptor Stable Cell Line
Documents
Document-MSDS: 10851_20090924043724.PDF (PDF)
TECHNICAL MANUAL: M00289_Manual.pdf (pdf)
Figures
CHO-K1/H4/Gα15 Stable Cell Line zoomCHO-K1/H4/Gα15 Stable Cell Line
Species
Human
Description
Histamine receptor family consists of four members, namely, H1, H2, H3, and H4. The histamine H4 receptor is the most recently identified G protein-coupled histamine receptor that binds to several neuroactive drugs, including amitriptyline and clozapine. So far, H4 receptors have been found only on haematopoietic cells. This signifies its important role as a drug target for the treatment of inflammatory diseases.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Application
Functional assay for H4 receptor
Application Examples
Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H4 receptor agonist, Histamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on H4 co-expressing with Gα15 in CHO-K1 cells was 147 nM. The S/B of Histamine on H4 co-expressing with Gα15 in CHO-K1 cells was 39.
Document-COA
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