| Product Name |
CHO-K1/OPRK1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant κ-Opioid Receptor OPRK1 Stable Cell Line |
|
Species |
Human |
Description |
Opioid receptors and their endogenous peptide ligands play important roles in the reward and reinforcement of drugs such as heroin, cocaine, and alcohol. The κ-opioid receptor is a type of opioid receptor which binds the peptide opioid dynorphin as the primary endogenous ligand. κ-opioid receptors are widely distributed in the brain, spinal cord, and in pain neurons. They are associated with the risk for alcohol dependence. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery. |
Application |
Functional assay for OPRK1 receptor |
Application Examples |
Figure 1. U-50488-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRK1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a OPRK1 receptor agonist, U-50488. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of U-50488 (Mean ± SD, n = 2). The EC50 of U-50488 on OPRK1 co-expressing with Gα15 in CHO-K1 cells was 4.7 nM. The S/B of U-50488 on OPRK1 co-expressing with Gα15 in CHO-K1 cells was 24. |
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TECHNICAL MANUAL: 11187_20091030030646.PDF (PDF)
Document-EXAMPLE: 11189_20091030030815.JPG (JPG)