| Product Name |
CHO-K1/CMKLR1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Chemokine Receptor-like 1 Stable Cell Line |
|
Species |
Human |
Description |
The orphan receptor ChemR23 (also referred to as CMKLR1 or DEZ) is a G-protein coupled receptor (GPCR) with homology to neuropeptide and chemoattractant receptors. ChemR23 is also a Gαi-linked receptor expressed primarily by monocytes, MΦs, and plasmacytoid dendritic cells. CMKLR1 has been shown to be expressed by DC generated in vitro from monocytes, to mediate their migration to the proteolytically regulated chemoattractant chemerin, and CMKLR1 may be a key mediator of pDC recruitment from the blood into tissue sites enriched in active chemerin. |
Freeze Medium |
45% Ham's F12, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CMKLR1 receptor |
Application Examples |
Figure 1. Chemerin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CMKLR1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a CMKLR1 receptor agonist, Chemerin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Chemerin (Mean ± SD, n = 2). The EC50 of Chemerin on CMKLR1 co-expressing with Gα15 in CHO-K1 cells was 35 nM. The S/B of Chemerin on CMKLR1 co-expressing with Gα15 in CHO-K1 cells was 7. |
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TECHNICAL MANUAL: 11184_20091030030432.PDF (PDF)
Document-EXAMPLE: 11186_20091030030534.JPG (JPG)