Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/OPRM1/Gα15
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Cat. No.
Name
Size   
Price
Selected
M00304
2 vials
$11,600
Product Name CHO-K1/OPRM1/Gα15 Stable Cell Line
Full Name
Human Recombinant μ-Opioid Receptor OPRM1 Stable Cell Line
Documents
TECHNICAL MANUAL: 11181_20091030025917.PDF (PDF)
Document-MSDS: 11426_20091130201907.PDF (PDF)
Figures
CHO-K1/OPRM1/Gα15 Stable Cell Line zoomCHO-K1/OPRM1/Gα15 Stable Cell Line
Reference
Species
Human
Description
The µ-opioid receptor (oprm1) is the principal site of action in the brain by which morphine, other opiate drugs of abuse, and endogenous opioid peptides effect analgesia and alter mood. Opioid receptors belong to the rhodopsin family of G protein-coupled receptors (GPCRs). The three types of opioid receptors (μ, δ, and κ) have been shown to associate with each other in a homotypic or heterotypic fashion when expressed in heterologous cells. A member of the seven-transmembrane domain (TM) G protein-coupled receptor (GPCR) superfamily, the µ-opioid receptor modulates ion channels and second messenger effectors in an opioid agonist-dependent fashion that is reversible by the classic opiate antagonist naloxone.
Freeze Medium
45% Ham's F12, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for OPRM1 receptor
Application Examples
Figure 1. DAMGO-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRM1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a OPRM1 receptor agonist, DAMGO. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of DAMGO (Mean ± SD, n = 2). The EC50 of DAMGO on OPRM1 co-expressing with Gα15 in CHO-K1 cells was 0.32 nM. The S/B of DAMGO on OPRM1 co-expressing with Gα15 in CHO-K1 cells was 22.
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