| Product Name |
CHO-K1/FPRL1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Formyl Peptide Receptor Like-1 Receptor Stable Cell Line |
|
Species |
Human |
Description |
The FPR family members including FPR, formyl peptide receptor like-1 and -2 (FPRL1, FPRL2), are differently expressed in phagocytes with neutrophils expressing FPR and FPRL1 and monocytes/macrophages expressing all three. FPRL1 may constitute an additional molecular target for the development of therapeutic agents for Alzheimer's disease (AD). GenScript has created a cell line that expresses FPRL1 in the CHO-K1 expressing Gα15. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for FPRL1 receptor |
Application Examples |
Figure 1. W-peptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FPRL1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a FPRL1 receptor agonist, w-peptide. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of w-peptide (Mean ± SD, n = 2). The EC50 of w-peptide on FPRL1 co-expressing with Gα15 in CHO-K1 cells was 0.076 nM. The S/B of w-peptide on FPRL1 co-expressing with Gα15 in CHO-K1 cells was 11. |
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TECHNICAL MANUAL: 11178_20091030025543.PDF (PDF)
Document-EXAMPLE: 11180_20091030025759.JPG (JPG)