Catalog Products » Other Products » Stable Cell Lines » Human Recombinant H2 Histamine Receptor Stable Cell Line
Email to GenScript

Cat. No.
Product Name
M00306 CHO-K1/H2/Gα15 Stable Cell Line
2 vials
Product Name CHO-K1/H2/Gα15 Stable Cell Line
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3, and H4. Specifically, the histamine receptor H2 belongs to the rhodopsin-like family of G protein-coupled receptors. As an integral membrane protein, it stimulates gastric acid secretion and regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation.
CHO-K1/H2/Gα15 Stable Cell LineTECHNICAL MANUAL: M00306_Manual.pdf (pdf)
CHO-K1/H2/Gα15 Stable Cell LineDocument-MSDS: 11428_20091130202230.PDF (PDF)
CHO-K1/H2/Gα15 Stable Cell Line zoomCHO-K1/H2/Gα15 Stable Cell Line
Freeze Medium
45% culture medium,45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Liquid nitrogen immediately upon delivery.
Functional assay for H2 receptor
Application Examples
Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H2 receptor agonist, Histamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on H2 co-expressing with Gα15 in CHO-K1 cells was 15 nM. The S/B of Histamine on H2 co-expressing with Gα15 in CHO-K1 cells was 5.
Related Products
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.