Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/H2/Gα15
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Cat. No.
Name
Size   
Price
Selected
M00306
2 vials
$11,600
Product Name CHO-K1/H2/Gα15 Stable Cell Line
Full Name
Human Recombinant H2 Histamine Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 11175_20091030025104.PDF (PDF)
Document-MSDS: 11428_20091130202230.PDF (PDF)
Figures
CHO-K1/H2/Gα15 Stable Cell Line zoomCHO-K1/H2/Gα15 Stable Cell Line
Reference
Description
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3, and H4. Specifically, the histamine receptor H2 belongs to the rhodopsin-like family of G protein-coupled receptors. As an integral membrane protein, it stimulates gastric acid secretion and regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation.
Freeze Medium
45% culture medium,45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery.
Application
Functional assay for H2 receptor
Application Examples
Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H2 receptor agonist, Histamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on H2 co-expressing with Gα15 in CHO-K1 cells was 15 nM. The S/B of Histamine on H2 co-expressing with Gα15 in CHO-K1 cells was 5.
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