Catalog Products » Other Products » Stable Cell Lines » Human Recombinant Melatonin MT2 Receptor Stable Cell Line
Email to GenScript

Cat. No.
Product Name
M00312 CHO-K1/MT2/Gα15 Stable Cell Line
2 vials
Product Name CHO-K1/MT2/Gα15 Stable Cell Line
CHO-K1/MT2/Gα15 Stable Cell LineTECHNICAL MANUAL: M00312_Manual.pdf (pdf)
CHO-K1/MT2/Gα15 Stable Cell LineDocument-MSDS: 11430_20091130202533.PDF (PDF)
CHO-K1/MT2/Gα15 Stable Cell Line zoomCHO-K1/MT2/Gα15 Stable Cell Line
Melatonin is a neurohormone that plays a key role in the synchronisation of circadian and seasonal functions with cyclic environmental variations. In mammals, two melatonin receptors, MT1 and MT2, have been cloned. Activation of MT2 melatonin receptors phase shift circadian rhythms of neuronal firing in the suprachiasmatic nucleus, inhibit dopamine release in retina, induce vasodilation and inhibition of leukocyte rolling in arterial beds, and enhance immune responses.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Liquid nitrogen immediately upon delivery
Functional assay for MT2 receptor
Application Examples
Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT2/Gα15 and CHO-K1/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a MT2 receptor agonist, Melatonin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.3 nM. The S/B of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.
Related Products
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.