Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/MT2/Gα15
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Cat. No.
Name
Size   
Price
Selected
M00312
2 vials
$8,500
Product Name CHO-K1/MT2/Gα15 Stable Cell Line
Full Name
Human Recombinant Melatonin MT2 Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: M00312_Manual.pdf (pdf)
Document-MSDS: 11430_20091130202533.PDF (PDF)
Figures
CHO-K1/MT2/Gα15 Stable Cell Line zoomCHO-K1/MT2/Gα15 Stable Cell Line
Species
Human
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for MT2 receptor
Application Examples
Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT2/Gα15 and CHO-K1/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a MT2 receptor agonist, Melatonin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.3 nM. The S/B of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.
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