Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/MT2/Gα15
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Cat. No.
Name
Size   
Price
Selected
M00312
2 vials
$11,600
Product Name CHO-K1/MT2/Gα15 Stable Cell Line
Full Name
Human Recombinant Melatonin MT2 Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 11166_20091030022338.PDF (PDF)
Document-MSDS: 11430_20091130202533.PDF (PDF)
Figures
CHO-K1/MT2/Gα15 Stable Cell Line zoomCHO-K1/MT2/Gα15 Stable Cell Line
Reference
Species
Human
Description
Melatonin is a neurohormone that plays a key role in the synchronisation of circadian and seasonal functions with cyclic environmental variations. In mammals, two melatonin receptors, MT1 and MT2, have been cloned. Activation of MT2 melatonin receptors phase shift circadian rhythms of neuronal firing in the suprachiasmatic nucleus, inhibit dopamine release in retina, induce vasodilation and inhibition of leukocyte rolling in arterial beds, and enhance immune responses.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for MT2 receptor
Application Examples
Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT2/Gα15 and CHO-K1/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a MT2 receptor agonist, Melatonin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.3 nM. The S/B of Melatonin on MT2 co-expressing with Gα15 in CHO-K1 cells was 7.
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