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Cat. No.
 Name
Size   
Price
 Selected
M00314
2 vials
Product Name 293/GHRH/Gα15 Stable Cell Line
Full Name
 Human Recombinant Growth Hormone Releasing Hormone Receptor Stable Cell Line
Documents
Document-EXAMPLE: 12968_20100618020700.JPG (JPG)
TECHNICAL MANUAL: 13045_20100710010619.PDF (PDF)
Document-MSDS: 13205_20100813051753.PDF (PDF)
Figures
Reference
Species
 Human
Description
 The growth hormone releasing hormone receptor GHRH is Gs-coupled GPCRs expressed in the pituitary and kidney. GHRH receptor stimulates growth hormone gene transcription and release. GenScript's cloned human GHRH-expressing cell line is made in the 293 host.
Freeze Medium
 45% culture medium, 45% FBS, 10% DMSO
Culture Medium
 DMEM, 10% FBS, 200 μg/ml Zeocin, 200 μg/ml Hygromycin B, 500 μg/ml G418
Storage
 Liquid nitrogen immediately upon delivery
Application
 Functional assay for GHRH receptor
Application Examples
 Figure 1. GHRF (1-29)-induced concentration-dependent stimulation of intracellular calcium mobilization in 293/GHRH/Gα15 and 293 cells. The cells were loaded with Calcium-4 prior to stimulation with a GHRH receptor agonist, GHRF (1-29). The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of GHRF (1-29) (Mean ± SD, n = 2). The EC50 of GHRF (1-29) on GHRH co-expressing with Gα15 in 293 cells was 8.8 nM. The S/B of GHRF (1-29) on GHRH co-expressing with Gα15 in 293 cells was 11.
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