| Product Name |
CHO-K1/PTH1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Parathyroid Hormone Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
Parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor (PTHR1) is a G protein coupled receptor which mediates the actions of both amino-terminal PTH and PTHrP fragments. The most abundant expression of PTHR1 is found in renal tubular cells and in osteoblasts, where the PTH/PTHrP receptor mediates the endocrine actions of PTH, and in prehypertrophic chondrocytes of the metaphyseal growth plate, where it mediates the autocrine/paracrine actions of PTHrP. Intact PTH (PTH 1-84) is compounded by a peptide of 84 amino acids (AA), the amino-terminal sequence, constituted by the first 34 AA (N-terminal structure), is necessary for its action. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for PTHR1 receptor |
Application Examples |
Figure 1. PTH 1-34-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTH/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a PTH1 receptor agonist, PTH 1-34. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of PTH 1-34 (Mean ± SD, n = 2). The EC50 of PTH 1-34 on PTH1 co-expressing with Gα15 in CHO-K1 cells was 9.7 nM. The S/B of PTH 1-34 on PTH1 co-expressing with Gα15 in CHO-K1 cells was 10. |
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