| Product Name |
CHO-K1/OX2 Stable Cell Line |
Full Name |
Human Recombinant OX2 Orexin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Hypocretin (orexin) receptor 2, also known as OX2, is a human protein encoded by the HCRTR2 gene. Orexin A and orexin B are neuropeptides originally identified as endogenous ligands for OX2. Orexin neuropeptides are produced by a small group of neurons in the lateral hypothalamic and perifornical areas, a region that is classically implicated with the control of mammalian feeding behavior. Orexin neurons that project throughout the central nervous system to nuclei are known to be important in the control of feeding, sleep-wakefulness, neuroendocrine homeostasis, and autonomic regulation. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for OX2 receptor |
Application Examples |
Figure 1. Orexin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OX2 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an OX2 receptor agonist, Orexin A. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Orexin A (Mean ± SD, n = 2). The EC50 of Orexin A on OX2 in CHO-K1 cells was 8.9 nM. The S/B of Orexin A on OX2 in CHO-K1 cells was 544. |
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