| Product Name |
HEK293/5-HT2C Stable Cell Line |
Full Name |
Human Recombinant 5-HT2C Serotonin Receptor Stable Cell Line |
|
Species |
Human |
Description |
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5HT2C is expressed in the brain and spinal cord, especially the choroid plexus. 5HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders, such as depression, panic anxiety, OCD, bulimia, and obesity. GenScript's cloned human 5-HT2C expressing cell line is generated in the CHO-K1 host. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 300 mg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for 5-HT2C receptor |
Application Examples |
Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/5-HT2C and HEK293 cells. The cells were loaded with Calcium-4 prior to stimulation with a 5-HT2C receptor agonist, 5-HT. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SD, n = 2). The EC50 of 5-HT on 5-HT2C in HEK293 cells was 1.7 nM. The S/B of 5-HT on 5-HT2C in HEK293 cells was 6. |
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