| Product Name |
CHO-K1/CT/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Calcitonin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Numerous studies have demonstrated that the calcitonin receptor (CALCR) is a specific marker of osteoclast differentiation and that calcitonin can inhibit bone resorption in vitro and in vivo. Mice lacking calcitonin and calcitonin gene-related peptide (CGRP) have a high bone mass phenotype due to an increase in bone formation parameters. Expression of calcitonin (CT) and its receptor (CTR) generate survival, adhesion, pro-inflammatory, and pro-metastatic pathways. Moreover, data indicate a pivotal role for CT-CTR axis in advanced prostate cancer PC metastasis and may serve as a potential therapeutic target for advanced PC. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CALCR receptor |
Application Examples |
Figure 1. Human Calcitonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CALCR/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a CALCR receptor agonist, Human Calcitonin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Human Calcitonin (Mean ± SD, n = 2). The EC50 of Human Calcitonin on CALCR co-expressing with Gα15 in CHO-K1 cells was 6.0 nM. The S/B of Human Calcitonin on CALCR co-expressing with Gα15 in CHO-K1 cells was 38. |
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