Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/D5/Gα15 Stable Cell Line
Cat. No.
Name
Size   
Price
Selected
M00322
2 vials
$11,600
Product Name CHO-K1/D5/Gα15 Stable Cell Line
Full Name
Human Recombinant D5 Dopamine Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 12990_20100627233212.PDF (PDF)
Document-MSDS: 13207_20100813051951.PDF (PDF)
Figures
CHO-K1/D5/Gα15 Stable Cell Line zoomCHO-K1/D5/Gα15 Stable Cell Line
Species
Human
Description
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). D5 receptor has a 10-fold higher affinity for dopamine than the D1 receptor.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for D5 receptor
Application Examples
Figure 1. Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D5/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D5 receptor agonist, Dopamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Dopamine (Mean ± SD, n = 2). The EC50 of Dopamine on D5 co-expressing with Gα15 in CHO-K1 cells was 6.5 nM. The S/B of Dopamine on D5 co-expressing with Gα15 in CHO-K1 cells was 24.
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