| Product Name |
CHO-K1/OPRD1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant δ-Opioid Receptor OPRD1 Stable Cell Line |
|
Species |
Human |
Description |
Opioid receptor family includes three classic receptors, μ, δ, and κ, also known as OP1, OP2 and OP3, respectively. The receptors are Gi/o-coupled GPCRs which will reduce intracellular cAMP levels after activation. δ-opioid receptor modulates many kinase cascades including ERKs, Akts, JNKs, STAT3, P38 involving Src, Ras, Rac, Raf-1, Cdc42, RTKs. In addition, δ-opioid receptor has also been proposed to interact with μ receptors. The observed pharmacological cross-talk may partially arise from agonist cross-reactivity. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for OPRD1 receptor |
Application Examples |
Figure 1. SNC80-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRD1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an OPRD1 receptor agonist, SNC80. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of SNC80 (Mean ± SD, n = 2). The EC50 of SNC80 on OPRD1 co-expressing with Gα15 in CHO-K1 cells was 18 nM. The S/B of SNC80 on OPRD1 co-expressing with Gα15 in CHO-K1 cells was 19. |
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