| Product Name |
CHO-K1/ADORA1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Adenosine A1 Receptor Stable Cell Line |
|
Species |
Human |
Description |
A1 is a receptor for adenosine. It is demonstrated that the A1 receptor is ubiquitous throughout the entire body. Activation of A1 elicits an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration. A1 receptors are implicated in sleep promotion by inhibiting wake promoting cholinergic neurons in the basal forebrain. Adenosine antagonists are widely used in neonatal medicine. |
Host Cell |
CHO-K1/Gα15 |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin, |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADORA1 receptor |
Application Examples |
Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADORA1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a ADORA1 receptor agonist, NECA. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of NECA (Mean ± SD, n = 2). The EC50 of NECA on ADORA1 co-expressing with Gα15 in CHO-K1 cells was 84 nM. The S/B of NECA on ADORA1 co-expressing with Gα15 in CHO-K1 cells was 14. |
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TECHNICAL MANUAL: 20091129194743 (PDF)
