| Product Name |
CHO-K1/ADORA2B/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Adenosine A2B Receptor Stable Cell Line |
|
Species |
Human |
Description |
The adenosine receptors ADORA2B is Gs and Gq/11-coupled GPCR expressed in the Large intestine, cecum, urinary bladder. ADORA2B receptor mediates relaxation to adenosine in human small coronary arteries which is independent of NO but dependents in part on a K+-sensitive mechanism. Pharmacological or molecular biologic activation of ADORA2B receptor may prevent glomerular remodeling associated with glomerulosclerosis, renal disease, and abnormal growth associated with hypertension and diabetes. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADORA2B receptor |
Application Examples |
Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADORA2B/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an ADOPA2B receptor agonist, NECA. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of NECA (Mean ± SD, n = 2). The EC50 of NECA on ADORA2B co-expressing with Gα15 in CHO-K1 cells was 14 nM. The S/B of NECA on ADORA2B co-expressing with Gα15 in CHO-K1 cells was 5. |
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