| Product Name |
CHO-K1/H3/Gα15 Stable Cell Line |
Full Name |
Human Recombinant H3 Histamine Receptor Stable Cell Line |
|
Species |
Human |
Description |
The histamine receptor H3 is a Gi-coupled GPCR expressed in the thalamus, caudate nucleus, putamen, cerebellum, amygdala, substantia nigra, hippocampus, hypothalamus and cerebral cortex. Activation of H3 receptor in human nasal mucosa inhibits sympathetic vasoconstriction. Expression of human H3 receptor splice variants in CHO cells shows that a deletion in the 2nd transmembrane domain alters ligand binding and deletions in the 3rd intracellular loop result in signal transduction being abolished. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for H3 receptor |
Application Examples |
Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H3/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an H3 receptor agonist, Histamine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on H3 co-expressing with Gα15 in CHO-K1 cells was 12 nM. The S/B of Histamine on H3 co-expressing with Gα15 in CHO-K1 cells was 8. |
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