Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/SST4/Gα15 Stable Cell Line
Cat. No.
Name
Size   
Price
Selected
M00334
2 vials
$11,600
Product Name CHO-K1/SST4/Gα15 Stable Cell Line
Full Name
Human Recombinant SST4 Somatostatin Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: M00334_Manual.pdf (pdf)
Document-MSDS: 13249_20100815220453.PDF (PDF)
Figures
CHO-K1/SST4/Gα15 Stable Cell Line zoomCHO-K1/SST4/Gα15 Stable Cell Line
Species
Human
Description
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST4 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in fetal and adult brain and lung. SST4 enhances AMPA receptor-mediated currents in the hippocampus, and interacts with the SST2 receptor.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for SST4 receptor
Application Examples
Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an SST14 receptor agonist, Somatostatin-14. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on SST4 co-expressing with Gα15 in CHO-K1 cells was 16 nM. The S/B of Somatostatin-14 on SST4 co-expressing with Gα15 in CHO-K1 cells was 18.
Document-COA
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