Catalog Products » Other Products » Stable Cell Lines » Human Recombinant SST4 Somatostatin Receptor Stable Cell Line
Email to GenScript

Cat. No.
Product Name
Size
Price
Quantity
M00334 CHO-K1/SST4/Gα15 Stable Cell Line
2 vials
$8500.00
Product Name CHO-K1/SST4/Gα15 Stable Cell Line
Species
Human
Documents
CHO-K1/SST4/Gα15 Stable Cell LineTECHNICAL MANUAL: M00334_Manual.pdf (pdf)
CHO-K1/SST4/Gα15 Stable Cell LineDocument-MSDS: 13249_20100815220453.PDF (PDF)
Figures
CHO-K1/SST4/Gα15 Stable Cell Line zoomCHO-K1/SST4/Gα15 Stable Cell Line
Description
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST4 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in fetal and adult brain and lung. SST4 enhances AMPA receptor-mediated currents in the hippocampus, and interacts with the SST2 receptor.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for SST4 receptor
Application Examples
Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an SST14 receptor agonist, Somatostatin-14. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on SST4 co-expressing with Gα15 in CHO-K1 cells was 16 nM. The S/B of Somatostatin-14 on SST4 co-expressing with Gα15 in CHO-K1 cells was 18.
Document-COA
Related Products
Cat. No.
Product Name
Size
Price
Quantity
RP10222-5 Octreotide (SMS 201-995)
5 mg
$119.00
RP10226 Somatostatin
5 mg
$57.00
RP10230 Somatostatin 28 (1-14)
1 mg
$81.00
1
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
2
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
3
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
4
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.