| Product Name |
293/ADRB3/CRE-Luc Stable Cell Line |
Full Name |
Human Recombinant ADRB3 Adrenoceptors Stable Cell Line |
|
Species |
Human |
Description |
The adrenoceptors ADRB3 is Gs-coupled GPCRs expressed in the adipose, gall bladder, small intestine, stomach, prostate, left atrium and bladder. The Trp64 -> Arg polymorphism is associated with earlier onset of non-insulin-dependent diabetes mellitus and reduced metabolic rate. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 50 μg/ml Hygromycin B, 100 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for ADRB3 receptor |
Application Examples |
Figure 1. Isoproterenol-induced concentration-dependent stimulation of intracellular cAMP in 293/ADRB3/CRE-Luc and 293/CRE-Luc cells. After stimulation with ADRB3 receptor agonist, isoproterenol, the cells are checked with Bright-GloTM Luciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10-fold dilution) of isoproterenol (Mean ± SD, n = 2). The EC50 of Isoproterenol on ADRB3 co-expressing with CRE-Luc in 293 cells was 21 nM. The S/B of isoproterenol on ADRB3 co-expressing with CRE-Luc in 293 cells was 90. |
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Document-MSDS: 13398_20101017210110.PDF (PDF)
Document-EXAMPLE: 12964_20100617234558.JPG (JPG)