| Product Name |
293/MC3/CRE-Luc Stable Cell Line |
Full Name |
Human Recombinant MC3 Melanocortin Receptor Stable Cell Line |
|
Species |
Human |
Description |
The melanocortin receptor 3 (MC3 receptor) is Gs-coupled GPCRs expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. MC3 receptor knockouts exhibit a metabolic syndrome. At 4-6 months old, MC3 receptor knockout mice show increased fat mass, reduced lean mass and a higher feed efficiency, with normal metabolic rates. MC3 knockout mice are hyperleptinaemic and males are often mildly hyperinsulinaemic. GenScript's cloned human MC3R-expressing cell line is made in the 293/CRE-Luciferase host. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 50 μg/ml Hygromycin B, 100 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for MC3 receptor |
Application Examples |
Figure 1. Drug-induced concentration-dependent stimulation of intracellular cAMP in 293/MC3/CRE-Luc cells. After stimulation with MC3 receptor agonist, ACTH and NDP-α-MSH, the cells are checked with Bright-GloTM Luciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10-fold dilution) of ACTH and NDP-α-MSH (Mean ± SD, n = 2). The EC50 of ACTH and NDP-α-MSH on MC3 co-expressing with CRE-Luc in 293 cells was 0.18 nM and 8.7 nM, respectively. The S/B of ACTH and NDP-α-MSH on MC3 co-expressing with CRE-Luc in 293 cells was 21. |
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