293/MC3/CRE-Luc Stable Cell Line

*This product has been discontinued!*

Recombinant HEK293 cells stably overexpress human melanocortin 3 receptor (MC3R) on the surface and contain high levels of G protein Gαs to couple with the inducible luciferase in downstream signaling pathways.

Cat. No.	M00337
Size	Buy in bulk

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Product Introduction Culture Conditions

Product Introduction

Product Description	Recombinant HEK293 cells stably overexpress human melanocortin 3 receptor (MC3R) on the surface and contain high levels of G protein Gαs to couple with the inducible luciferase in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	DMEM, 10% FBS, 100 μg/ml Zeocin (Cat. No. 460509, Life Technologies), 50 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	45% FBS (Cat. No. A5669701, Gibco), 45% DMEM (Cat. No. 10569-010 Gibco) ,10% DMSO (Cat. No. D2650, Sigma)

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