| Product Name |
CHO-K1/NPS1a/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Neuropeptide S Receptor Isoform A Stable Cell Line |
|
Species |
Human |
Description |
The neuropeptide S receptor isoform a (NPS1a) is Gq/11 and Gs-coupled GPCRs expressed in the bronchial smooth muscle cells, basally in colon epithelium and in occasional basal keratinocytes in skin. An Asn107->Ile mutation is significantly up-regulated in a mouse model of ovalbumen-induced lung inflammation supporting a role in the pathogenesis of asthma. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Application |
Functional assay for NPS1a receptor |
Application Examples |
Figure 1. NPS-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPS1a/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an NPS1a receptor agonist, NPS. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of NPS (Mean ± SD, n = 2). The EC50 of NPS on NPS1a co-expressing with Gα15 in CHO-K1 cells was 9.8 nM. The S/B of NPS on NPS1a co-expressing with Gα15 in CHO-K1 cells was 33. |
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