CHO-K1/NPS1a/Gα15 Stable Cell Line

The neuropeptide S receptor isoform a (NPS1a) is Gq/11 and Gs-coupled GPCRs expressed in the bronchial smooth muscle cells, basally in colon epithelium and in occasional basal keratinocytes in skin. An Asn107->Ile mutation is significantly up-regulated in a mouse model of ovalbumen-induced lung inflammation supporting a role in the pathogenesis of asthma.

Price	$8500.00
Cat. No.	M00338
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human neuropeptide S receptor 1 (NPSR1) isoform a on the surface and contain high levels of G protein Gαq and Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

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Figure 1. Neuropeptide S-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPS1a/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with NPSR1 agonist, neuropeptide S (NPS). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of NPS (mean ± SEM, n = 3). The EC50 of NPS on CHO-K1/NPS1a/Gα15 cells was 98.48 ng/ml.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.