Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/ADRA1D Stable Cell Line
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Cat. No.
Name
Size   
Price
Selected
M00340
2 vials
$8,500
Product Name CHO-K1/ADRA1D Stable Cell Line
Full Name
Human Recombinant ADRA1D Adrenoceptors Stable Cell Line
Documents
TECHNICAL MANUAL: M00340_Manual.pdf (pdf)
Document-MSDS: 13244_20100815220112.PDF (PDF)
Figures
CHO-K1/ADRA1D Stable Cell Line zoomCHO-K1/ADRA1D Stable Cell Line
Species
Human
Freeze Medium
45% culture medium, 45%FBS, and 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 400 μg/ml G418
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assays for ADRA1D receptors
Application Examples
Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1D and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a ADRA1D receptor agonist, Epinephrine. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Epinephrine (Mean ± SD, n = 2). The EC50 of Epinephrine on ADRA1D in CHO-K1 cells was 7.1 nM. The S/B of Epinephrine on ADRA1D in CHO-K1 cells was 21.
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